In our previous videos we introduced the CRISPR Cas9 system briefly (
http://youtu.be/1aJxXWkE3Ek) and presented the different tools and methods available for their expression in living systems (
http://youtu.be/INC_kdr7I34). Here we will go over the design criteria of the gRNA and introduce you to the online programs that simplify this procedure. We invite you to watch our previous videos first before continuing with this one.
For more information on how to design gRNA for CRISPR Cas9 systems, please visit:
➜ Knowledge Base:
https://info.abmgood.com/crispr-cas9-sgrna-designChop Chop Harvard:
https://chopchop.rc.fas.harvard.edu/*Please note: There is an error at ~1:12. Cas9 has an HNH and an RuvC1-like nuclease domain (in the video, it was mislabeled as "NHN").
Want more tips, tricks, guides, and protocols on how to knockout a gene using CRISPR? Join our free, 4-week #CRISPRCrashCourse:
http://info.abmgood.com/crispr-crash-courseFor more resources on CRISPR, visit:
➜
https://info.abmgood.com/crispr-cas9*Spanish subtitles are also available
Watch the other videos in this series on CRISPR:
➜ Introduction:
https://youtu.be/1aJxXWkE3Ek➜ Methods and Tools:
https://youtu.be/INC_kdr7I34➜ CRISPR Playlist:
https://youtu.be/1aJxXWkE3Ek?list=PLTt9kKfqE_0Ei8_rQsrfm01-zQtABTn0ZCheck out our other video series:
➜ Adeno Associated Virus:
https://youtu.be/hYHbfQe5h-Q?list=PLTt9kKfqE_0HfXQMX9RPgbcmSWMqVtlBf➜ Next Generation Sequencing:
https://youtu.be/jFCD8Q6qSTM?list=PLTt9kKfqE_0Gem8hIcJEn7YcesuuKdt_nConnect with us on our social media pages to stay up to date with the latest scientific discoveries:
➜ Facebook:
https://goo.gl/hc9KrG➜ Twitter:
https://goo.gl/gGGtT9➜ LinkedIn:
https://goo.gl/kSmbht➜ Google+:
https://goo.gl/5bRNwCAbout the Site 🌐
This site provides links to random videos hosted at YouTube, with the emphasis on random. 🎥
Origins of the Idea 🌱
The original idea for this site stemmed from the need to benchmark the popularity of a video against the general population of YouTube videos. 🧠
Challenges Faced 🤔
Obtaining a large sample of videos was crucial for accurate ranking, but YouTube lacks a direct method to gather random video IDs.
Even searching for random strings on YouTube doesn't yield truly random results, complicating the process further. 🔍
Creating Truly Random Links 🛠️
The YouTube API offers additional functions enabling the discovery of more random videos. Through inventive techniques and a touch of space-time manipulation, we've achieved a process yielding nearly 100% random links to YouTube videos.
About YouTube 📺
YouTube, an American video-sharing website based in San Bruno, California, offers a diverse range of user-generated and corporate media content. 🌟
Content and Users 🎵
Users can upload, view, rate, share, and comment on videos, with content spanning video clips, music videos, live streams, and more.
While most content is uploaded by individuals, media corporations like CBS and the BBC also contribute. Unregistered users can watch videos, while registered users enjoy additional privileges such as uploading unlimited videos and adding comments.
Monetization and Impact 🤑
YouTube and creators earn revenue through Google AdSense, with most videos free to view. Premium channels and subscription services like YouTube Music and YouTube Premium offer ad-free streaming.
As of February 2017, over 400 hours of content were uploaded to YouTube every minute, with the site ranking as the second-most popular globally. By May 2019, this figure exceeded 500 hours per minute. 📈
List of ours generators⚡
Random YouTube Videos Generator
Random Film and Animation Video Generator
Random Autos and Vehicles Video Generator
Random Music Video Generator
Random Pets and Animals Video Generator
Random Sports Video Generator
Random Travel and Events Video Generator
Random Gaming Video Generator
Random People and Blogs Video Generator
Random Comedy Video Generator
Random Entertainment Video Generator
Random News and Politics Video Generator
Random Howto and Style Video Generator
Random Education Video Generator
Random Science and Technology Video Generator
Random Nonprofits and Activism Video Generator