Transcription termination in prokaryotes – This lecture explains about the prokaryotic transcription termination. RNA synthesis will continue along the DNA template strand until the polymerase encounters a sign that tells it to stop, or terminate, transcription. In prokaryotes, this signal can take two types, rho-unbiased and rho-dependent.
Rho-independent Terminator
The rho- independent terminator is the extra simple of the two programs and thus is also referred to as easy termination. The rho- independent signal is located on the DNA template strand and consists of a neighborhood that contains a section that is then repeated a number of base pairs away in the inverted sequence.
When this stretch is transcribed into an RNA sequence, the RNA can fold back and base pair with itself forming a hairpin loop. As you can find, the string of adenines in the DNA sequence are transcribed into uracils within the RNA sequence. On account that the uracil bases will simplest pair weakly with the adenines, the RNA chain can conveniently be launched from the DNA template, terminating transcription.
Rho-dependent Terminator
The rho- dependent terminator acquired its title in view that it is elegant on a particular protein called a rho component. The rho component is idea to bind to the end of the RNA chain and slide along the strand closer to the open tricky bubble. When the aspect catches the polymerase, it motives the termination of transcription. The mechanism of this termination is uncertain; however the rho component would by some means pull the polymerase tricky off of the DNA strand.
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