📌Link article:
https://dimedinet.com/category/tecnicas-biologia-molecular/This video demonstrates how to load and run DNA samples on an agarose gel. Basic information about the charge of DNA and how it will run in an horizontal electrophoresis cell is explained.
I hope you enjoy and learn from it. If you have any questions leave a comment. Subscribe if you think this was helpfull =)
Here, some final considerations:
- The porosity of agarose gel depends on its concentration in TE buffer solution.
- The DNA phosphate groups to possess has a negative charge. Therefore, by making the size electrophoresis and no load should be considered.
- When you have a ratio of absorbance (260/280) of less than 2 protein contamination exists and if greater phenol, chloroform or carbohydrates.
- When you have a ratio of absorbance (260/280) of approximately 2 it indicates that we have a pure sample.
- After electrophoresis and observed through UV light, the sample DNA showed increased migration depending on the size of their molecular weights
- While the absorbance is related to concentration, it is not as such.
TITLE IN YOUTUBE:
THE BEST EXPLANATION OF DNA GEL ELECTROPHORESIS | EXPLICACION PASO A PASO ELECTROFORESIS DE ADN EN GEL DE AGAROSA
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TERMINOS DE BUSQUEDA RELACIONADOS CON LA TEMATICA:
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Transfer DNA with pipette,
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